Molecular breeding for the genetic improvement of forage crops and turf : proceedings of the 4. international symposium on the molecular breeding of forage and turf, a satellite workshop of the 20. International Grassland Congress, 2005, Aberystwyth, Wales
Provides an account of progress and potential in the genetic improvement of grassland to meet all needs. This book includes work on a range of temperate and tropical grassland species, and is of interest to those concerned with grassland use in livestock-based agriculture, recreation, environmental protection, bio-industry, and more.
Print Book, English, 2005
Wageningen Academic Publishers, Wageningen, 2005
286 s. : illustrations ; 24 cm
Foreword 7; Keynote presentations 17; Objectives and benefits of molecular breeding in forage species 19; T. Lubberstedt; Introgression mapping in the grasses 31; I.P. King, J. King, I.P. Armstead, J.A. Harper, L.A. Roberts, H. Thomas, H.J. Ougham, R.N. Jones, A. Thomas, BJ Moore, L. Huang and I.S. Donnison; QTL analysis and trait dissection in ryegrasses (Lolium spp.) 43; T. Yamada and J.W. Forster; Translational genomics for alfalfa varietal improvement 55; G.D. May; Application of molecular technologies in forage plant breeding 63; K.F. Smith, J.W. Forster, M.P. Dobrowolski, N.O.I. Cogan, N.R. Bannan, E. van Zijll de Jong, M. Emmerling and G.C. Spangenberg; A computational pipeline for the development of comparative anchor tagged sequence (CATS) markers 73; L. Schauser, J. Fredslund, L. Heegaard Madsen, N. Sandal and J. Stougaard; Future directions in the molecular breeding of forage and turf 83; G.C. Spangenberg, J.W. Forster, D. Edwards, U. John, A. Mouradov, M. Emmerling, J. Batley, S. Felitti, N.O.I. Cogan, K.F. Smith and M.P. Dobrowolski; Application of molecular markers in genetic resources management of perennial ryegrass 99; R. van Treuren; Section 1: Objectives, benefits and targets of molecular breeding 111; Leaves of high yielding perennial ryegrass contain less aggregated Rubisco than S23 113; A. Kingston-Smith and P.W. Wilkins; Variability in quantity and composition of water soluble carbohydrates among Irish accessions and European varieties of perennial ryegrass 114; S. McGrath, S. Barth, A. Frohlich, M. Francioso, S.A. Lamorte and T.R. Hodkinson; Introgression breeding for improvement of winter hardiness in Lolium/Festuca complex using androgenenesis 115; T. Yamada, Y.D. Guo and Y. Mizukami; A new napier grass stunting disease in Kenya associated with phytoplasma 116; A.B. Orodho, S.I. Ajanga, P. Jones and P.O. Mudavadi; Studies of seed characteristics of ecotypes of lucerne, Bromus and Agropyron in response to Fusarium oxysporum and F. solani 117; M.A. Alizadeh; Genetic analysis of the interaction between the host perennial ryegrass and the crown rust pathogen (Puccinia coronata f.sp. lolii) 118; P.M. Dracatos, J.L. Dumsday, R.S. Olle, N.O.I. Cogan, M.P. Dobrowolski, K.F. Smith and J.W. Forster; Molecular characterisation of bacterial wilt resistance in Lolium multiflorum Lam. 119; B. Studer, B. Boller, F. Widmer, U.K. Posselt, E. Bauer and R. Kolliker; Discriminating stay-green grasses using hyperspectral imaging and chemometrics 120; J. Taylor, B. Moore, J.J. Rowland, H. Thomas and H. Ougham; Non-destructive assessment of quality and yield for grass-breeding 121; A.G.T. Schut, M.J.J. Pustjens, P. Wilkins, J. Meuleman, P. Reyns, A. Lovatt and G.W.A.M. van der Heijden; Root senescence in red clover (Trifolium pratense L.) 122; K.J. Webb, E. Tuck and S. Heywood; Tropical vine legume-maize mixtures for enhanced silage in temperate climates 123; H. Riday. Section 2: Linkage/physical mapping and map-based cloning of genes 125; Development of simple sequence repeat (SSR) markers and their use to assess genetic diversity in apomictic Guineagrass (Panicum maximum Jacq.) 127; M. Ebina, K. Kouki, S. Tsuruta, M. Takahara, M. Kobayashi, T. Yamamoto, K. Nakajima and H. Nakagawa 127; Construction of microsatellite-enriched libraries for tropical forage species and characterization of the repetitive sequences found in Brachiaria brizantha 128; L. Jungmann, C.B. do Valle, P.R. Laborda, R.M.S. Resende, L. Jank and A.P. de Souza; Isolation of SSR markers from Zoysiagrass 129; H. Cai, N. Yuyama and M. Inoue; Development of SSR markers for variety identification in Italian ryegrass (Lolium multiflorum Lam.) 130; M. Inoue, N. Yuyama and H. Cai; Development of EST-derived simple sequence repeat (SSR) markers in Italian ryegrass (Lolium multiflorum Lam.) 131; M. Hirata, Y. Miura, T. Takamizo and M. Fujimori; Development of a microsatellite library in Lolium perenne 132; J. King, I.P. King, D. Thorogood, L. Roberts, K. Skot and K. Elborough; Tall fescue expressed sequence tag and simple sequence repeats: important resources for grass species 133; M.C. Saha, J.C. Zwonitzer, K. Chekhovskiy and M.A.R. Mian; Development of EST and AFLP markers linked to a gene for resistance to ryegrass blast (Pyricularia sp.) in Italian ryegrass (Lolium multiflorum Lam.) 134; Y. Miura, C. Ding, R. Ozaki, M. Hirata, M. Fujimori, H. Cai and K. Mizuno; Construction and exploitation of a bacterial artificial chromosome (BAC) library for Lolium perenne (perennial ryegrass) 135; K. Farrar, A.M. Thomas, M.O. Humphreys and I.S. Donnison; Characterisation of perennial ryegrass parental inbred lines for generating recombinant inbred lines for fine mapping and gene cloning 136; U.C.M. Anhalt, S. Barth, T. Schwarzacher and J.S. Heslop-Harrison; A high-density SSR linkage map of red clover and its transferability to other legumes 137; S. Isobe, S. Sato, E. Asamizu, I. Klimenko, N.N. Kozlov, K. Okumura and S. Tabata; Estimation of the coefficient of double-reduction in autotetraploid lucerne 138; R. Ayadi, P. Barre, C. Huyghe and B. Julier; A core AFLP map of aposporic tetraploid Paspalum notatum (Bahiagrass) 139; J.P.A. Ortiz, J. Stein, E.J. Martinez, S.C. Pessino and C.L. Quarin; Repulsion-phase linkage analysis of tetraploid creeping bentgrass (Agrostis stolonifera L.) 140; S.E. Warnke, N. Chakraborty and G. Jung; Towards a genetic map in creeping bentgrass based on SSRs, AFLPs and RFLPs 141; H. Zhao and S. Bughrara; Integration of perennial ryegrass (L. perenne) genetic maps using gene-associated SNPs 142; A.C. Vecchies, R.C. Ponting, M.C. Drayton, N.O.I. Cogan, K.F. Smith, G.C. Spangenberg and J.W. Forster; Construction and comparison of genetic linkage maps of four F1 populations of Italian ryegrass (Lolium multiflorum Lam.) 143; M. Vandewalle; Section 3: QTL analysis and trait dissection 145; QTLs for morphogenetic traits in Medicago truncatula 147; B. Julier, T. Huguet, J.M. Prosperi, P. Barre, G. Cardinet and C. Huyghe; A Medicago truncatula population segregating for aluminum tolerance 148; M. Sledge, B. Narasimhamoorthy and G. Jiang; Genetic mapping in tetraploid alfalfa: Results and prospects 149; E.C. Brummer, J.G. Robins, B. Alarcon Zuniga and D. Luth; Quantitative trait locus analysis of morphogenetic and developmental traits in an SSR- and AFLP-based genetic map of white clover (Trifolium repens L.) 150; M.T Abberton, N.O.I. Cogan, K.F. Smith, G. Kearney, A.H. Marshall, A. Williams, T.P.T. Michaelson-Yeates, C. Bowen, E.S. Jones, A.C. Vecchies and J.W. Forster. Changes in gene expression during acclimation to cold temperatures in white clover (Trifolium repens L.) 151; M. Lowe, R.P. Collins and M.T. Abberton; QTL analysis of mineral content and grass tetany potential in Leymus wildryes 152; S.R. Larson and H.F. Mayland; QTL analysis of mineral content in perennial ryegrass (Lolium perenne L.) 153; N.O.I. Cogan, A.C. Vecchies, T. Yamada, K.F. Smith and J.W. Forster; A glucanase gene cosegregates with a QTL for crown rust resistance in L. perenne 154; H. Muylle, J. Baert, E. Van Bockstaele and I. Roldan-Ruiz; Mapping water-soluble carbohydrate content in perennial ryegrass 155; L. Turner, J. Gallagher, I. Armstead, A. Cairns and M. Humphreys; Quantitative trait loci for vegetative traits in perennial ryegrass (Lolium perenne L.) 156; A.M. Sartie, H.S. Easton, M.J. Faville and C. Matthew; Approaches for associating molecular polymorphisms with phenotypic traits based on linkage disequilibrium in natural populations of Lolium perenne 157; L. Skot, J. Humphreys, I.P. Armstead, M.O. Humphreys, J.A. Gallagher and I.D. Thomas; Identification of quantitative trait loci for flowering time in a field-grown Lolium perenne x Lolium multiflorum mapping population 158; R.N. Brown, R.E. Barker, S.E. Warnke, L.A. Brilman, M.A.R. Mian, S.C. Sim and G. Jung; Crown rust resistance of Italian ryegrass cultivar 'Axis' to an isolate from Japan 159; T. Kiyoshi, M. Hirata, T. Takamizo, H. Sato, Y. Mano and M. Fujimori; Locating, and utilising Festuca pratensis genes for winter hardiness for the future development of more persistent high quality Lolium cultivars 160; M.W. Humphreys, D. Gasior, A. Kosmala, O.A. Rognli, Z. Zwierzykowski and M. Rapacz; QTL analysis of vernalisation requirement and heading traits in Festuca pratensis Huds. 161; A. Ergon, C. Fang, O. Jorgensen, T.S. Aamlid and O.A. Rognli; Consistency of QTL for dollar spot resistance between greenhouse and field inoculations, multiple locations, and different population sizes in creeping bentgrass 162; N. Chakraborty, J. Bae, J. Curley, S. Warnke, M. Casler, S. Bughrara and G. Jung; Section 4: Genomics, model species, gene discovery and functional analysis 163; Structural and functional genomic research in model legume plants: The National BioResource Project (NBRP) in Japan 165; S. Tsuruta, M. Hashiguchi and R Akashi; Identification of putative AtTT2 R2R3-MYB transcription factor orthologues in tanniferous tissues of L. corniculatus var. japonicus cv Gifu 166; D.N Bryant, P. Bailey, P. Morris, M. Robbins, C. Martin and T. Wang; Foliar expression of candidate genes involved in condensed tannin biosynthesis in white clover (Trifolium repens) 167; S.N. Panter, J. Simmonds, A. Winkworth, A. Mouradov and G.C. Spangenberg; Discovery, isolation and characterisation of promoters in white clover (Trifolium repens) 168; C.M. Labandera, Y.H. Lin, E. Ludlow, M. Emmerling, U. John, P.W. Sale, C. Pallaghy and G.C. Spangenberg; Application of molecular markers derived from Medicago truncatula in white clover (Trifolium repens L.) 169; C. Jones and M.T. Abberton; Gene-associated single nucleotide polymorphism discovery in white clover (T. repens L.) 170; M.C. Drayton, R.C. Ponting, A.C. Vecchies, T.C. Wilkinson, J. George, N.O.I. Cogan, N.R. Bannan, K.F. Smith, G.C. Spangenberg and J.W. Forster; A molecular study of alfalfa megasporogenesis 171; D. Rosellini, S. Capomaccio and F. Veronesi; The efficacy of GeneThresher[registered] methylation filtering technology in the plant kingdom 172; U. Warek, J.A. Bedell, M.A. Budiman, A.N. Nunberg, R.W. Citek, D. Robbins, N. Lakey and P.D. Rabinowicz; Screening of perennial grasses and a mutant maize collection by Fourier-Transformed InfraRed (FTIR) spectroscopy for improved biofuel traits 173; S.C. Thain, P. Morris, S. Hawkins, C. Morris and I.S. Donnison. A proposal for an international transcriptome initiative for forage and turf: microarray tools for expression profiling in ryegrass, clover and grass endophytes 174; T. Webster, N. Nguyen, C. Rhodes, S. Felitti, R. Chapman, D. Edwards and G.C. Spangenberg; Isolation and characterisation of genes encoding malate synthesis and transport determinants in the aluminium-tolerant Australian weeping-grass (Microlaena stipoides) 175; R.M. Polotnianka, E. Ribarev, L. Mackin, K.A. Sivakumaran, G.D. Nugent, U.P. John and G.C. Spangenberg; Novel genotypes of the subtropical grass Eragrostis curvula for the analysis of apomixis (diplospory) 176; S. Cardone, P. Polci, J.P. Selva, M. Mecchia, S. Pessino, P. Voigt, G.C. Spangenberg and V. Echenique; Discovery and functional categorisation of expressed sequence tags from flowers of Eragrostis curvula genotypes showing different ploidy levels and reproductive modes 177; V. Echenique, S. Felitti, N. Paniego, L. Martelotto, S. Pessino, D. Zanazzi, P. Fernandez, M. Diaz, P. Polci and G.C. Spangenberg; A comprehensive analysis of gene expression and genomic alterations in a newly formed autotetraploid of Paspalum notatum 178; L.G. Martelotto, J.P.A. Ortiz, F. Espinoza, C.L. Quarin and S.C. Pessino; Gene discovery and molecular dissection of fructan metabolism in perennial ryegrass (Lolium perenne) 179; J. Chalmers, A. Lidgett, X. Johnson, K. Terdich, N. Cummings, Y.Y. Cao, K. Fulgueras, M. Emmerling, T. Sawbridge, E.K. Ong, A. Mouradov and G.C. Spangenberg; Gene discovery and molecular dissection of lignin biosynthesis in perennial ryegrass (Lolium perenne) 180; A. Lidgett, M. Emmerling, R. Heath, R. McInnes, D. Lynch, A. Bartkowski, K. Fulgueras, T. Sawbridge, E.K. Ong, K.F. Smith, A. Mouradov and G.C. Spangenberg; An in silico DNA sequence comparison of the perennial ryegrass and rice genomes 181; M.J. Faville; The identification of genetic synteny between Lolium perenne chromosome 7 and rice chromosome 6 genomic regions that have major effects on heading-date 182; I.P. Armstead, L.B. Turner, L. Skot, I.S. Donnison, M.O. Humphreys and I.P. King; Towards understanding photoperiodic response in grasses 183; M. Gagic, I. Kardailsky, N. Forester, B. Veit and J. Putterill; Controlled flowering project for Lolium perenne at Agresearch: an overview 184; I. Karadailsky, B. Veit, N. Forester, M. Gagic, K. Richardson, M. Faville and G. Bryan; The investigation of flowering control in late/rare flowering Lolium perenne 185; S. Byrne, I. Donnison, L.J. Mur and E. Guiney; Isolation of candidate genes involved in cold temperature response in Festuca pratensis Huds., using suppression subtractive hybridisation and microarray approaches 186; H. Rudi, V. Alm, L. Opseth, A. Larsen and O.A. Rognli; Isolation and characterization of a CBF gene from perennial ryegrass (Lolium perenne L.) 187; Y. Xiong and S. Fei; Isolation and characterisation of genes encoding ice recrystallisation inhibition proteins (IRIPs) in the cryophilic antarctic hair-grass (Deschampsia antarctica) and the temperate perennial ryegrass (Lolium perenne) 188; U.P. John, R.M. Polotnianka, K.A. Sivakumaran, L. Mackin, M.J. Kuiper, J.P. Talbot, O. Chew, G.D. Nugent, N.O.I. Cogan, M.C. Drayton, J.W. Forster, G.E. Schrauf and G.C. Spangenberg; Development of genetic markers for drought tolerance in Festuca-Lolium complexes 189; J.P. Wang and S.S. Bughrara; Monitoring of gene expression profiles and identification of candidate genes involved in drought tolerance in Festuca mairei with cDNA-AFLP 190; J.P. Wang and S.S. Bughrara; Section 5: Use of molecular markers and bioinformatics in breeding 191; Towards a comparative map of white clover (Trifolium repens) and barrel medic (Medicago truncatula) 193; M. Febrer, G. Jenkins, M. Abberton and D. Milbourne; Use of cross-species amplification markers for pollen-mediated gene flow determination in Trifolium polymorphum Poiret 194; M. Dalla Rizza, D. Real, R. Reyno and K. Quesenberry; Clover ASTRA: a web-based resource for Trifolium EST analysis 195; G.C. Spangenberg, T. Sawbridge, E.K. Ong, C.G. Love, T.A. Erwin, E.G. Logan and D. Edwards. SNP discovery and haplotypic variation in full-length herbage quality genes of perennial ryegrass (Lolium perenne L.) 196; R.C. Ponting, M.C. Drayton, N.O.I. Cogan, G.C. Spangenberg, K.F. Smith and J.W. Forster; Development and use of a tool for automated alignments of genes in the rice BAC's GenBank card against other species 197; P. Barre, G. Darrieutort, J. Auzanneau and B. Julier; Screening genes for association with loci for nitrogen-use efficiency in perennial ryegrass by pyrosequencing[trademark] 198; O. Dolstra, D. Dees, J.-D. Driesprong and E.N. van Loo; Gene-associated single nucleotide polymorphism (SNP) discovery in perennial ryegrass (Lolium perenne L.) 199; J.W. Forster, N.O.I. Cogan, A.C. Vecchies, R.C. Ponting, M.C. Drayton, J. George, J.L. Dumsday, G.C. Spangenberg and K.F. Smith; Development and testing of novel chloroplast markers for perennial ryegrass from de novo sequencing and in silico sequences 200; S. McGrath, T.R. Hodkinson and S. Barth; Ryegrass ASTRA: a web-based resource for Lolium EST analysis 201; G.C. Spangenberg, T. Sawbridge, E.K. Ong, C.G. Love, T.A. Erwin, E.G. Logan and D. Edwards; Positive effect of increased AFLP diversity among parental plants on yield of polycross progenies in perennial ryegrass (Lolium perenne L.) 202; R. Kolliker, B. Boller and F. Widmer; Genomic constitution of Festulolium varieties 203; D. Kopecky, V. Cernoch, R. Capka and J. Dolezel; Genetic changes over breeding generations of Festulolium 204; M. Ghesquiere, P. Barre and L. Barrot; Phenotypic variation within local populations of meadow fescue shows significant associations with allele frequencies at AFLP loci 205; S. Fjellheim, A.B. Blomlie, P. Marum and O.A. Rognli; Marker-assisted selection for fibre concentration in smooth bromegrass 206; C. Stendal, M.D. Casler and G. Jung; Endophyte ASTRA: a web-based resource for Neotyphodium and Epichloe EST analysis 207; K. Shields, M. Ramsperger, S.A. Felitti, C.G. Love, T.A. Erwin, D. Singh, E.G. Logan, D. Edwards and G.C. Spangenberg; Section 6: Genetics and breeding for symbiosis 209; Genetic variation in the perennial ryegrass fungal endophyte Neotyphodium lolii 211; E. van Zijll de Jong, N.R. Bannan, A.V. Stewart, K.F.M. Reed, M.P. Dobrowolski, K.F. Smith, G.C. Spangenberg and J.W. Forster; Isolation and characterisation of novel BTB domain protein encoding genes from fungal grass endophytes 212; M. Ramsperger, S.A. Felitti, D. Edwards and G.C. Spangenberg; Genetic analysis of the interaction between perennial ryegrass and the fungal endophyte Neotyphodium lolii 213; E. van Zijll de Jong, A.C. Vecchies, M.P. Dobrowolski, N.O.I. Cogan, K.F. Smith, G.C. Spangenberg and J.W. Forster; Microarray-based transcriptome analysis of the interaction between perennial ryegrass (Lolium perenne) and the fungal endophyte Neotyphodium lolii 214; S.A. Felitti, P. Tian, T. Webster, D. Edwards and G.C. Spangenberg; A high-throughput gene silencing approach for studying the interaction between perennial ryegrass (Lolium perenne) and the fungal endophyte Neotyphodium lolii 215; S.A. Felitti, P. Tian, D. Edwards and G.C. Spangenberg; Metabolome analysis of the interaction between perennial ryegrass (Lolium perenne) and the fungal endophyte Neotyphodium lolii 216; P. Tian, S.A. Felitti, M.P. Dobrowolski, K.F. Smith, D. Edwards, R. Hall, J. Kopka and G.C. Spangenberg; Endophyte effects on antioxidants and membrane leakage in tall fescue during drought 217; C.P. West, R.D. Carson, C.A. Guerber and B. de los Reyes. Section 7: Transgenics for research and breeding including risk assessment 219; Role of the BANYULS(BAN) gene from Arabidopsis thaliana in transgenic Alfalfa expression of anthocyanins and proanthocyanidins 221; S.M. Hesamzadeh Hejazi, S. Arcioni and F. Paolocci; Development of alfalfa (Medicago sativa L.) transgenic plants expressing a Bacillus thuringiensis endotoxin and their evaluation against alfalfa caterpillar (Colias lesbia) 222; F. Ardila, M.C. Gomez, M.J. Dieguez, E.M. Pagano, M. Turica, R. Lecuona, V. Arolfo, D. Basigalup, C. Vazquez Rovere, E. Hopp, P. Franzone and R.D. Rios; Increased cuticular wax accumulation and enhanced drought tolerance in transgenic alfalfa by overexpression of a transcription factor gene 223; Z.-Y. Wang, J.-Y. Zhang, C. Broeckling, E. Blancaflor, M. Sledge and L. Sumner; Molecular breeding of white clover for transgenic resistance to Alfalfa mosaic virus and natural resistance to Clover yellow vein virus 224; P. Chu, G. Zhao and G.C. Spangenberg; Molecular breeding of transgenic virus-immune white clover (Trifolium repens) cultivars 225; M. Emmerling, P. Chu, K.F. Smith, C. Binnion, M. Ponnampalam, P. Measham, Z.Y. Lin, N. Bannan, T. Wilkinson and G.C. Spangenberg; Polyphenolic phenomena: transgenic analysis of some of the factors that regulate the cell-specific accumulation of condensed tannins (proanthocyanidins) in forage crops 226; M.P. Robbins, G. Allison, D. Bryant and P. Morris; Minimising bloat through development of white clover (T. repens) with high levels of condensed tannins 227; M.T. O'Donoghue, C. Spillane and E. Guiney; Production and analysis of transgenic white clover (Trifolium repens) plants over-expressing organic acid biosynthetic genes 228; C.M. Labandera, S. Panter, A. Winkworth, J. Simmonds, A. Mouradov, U. John, P.W. Sale and G.C. Spangenberg; LXR[trademark] white clover: development of transgenic white clover (Trifolium repens) with delayed leaf senescence 229; Y.H. Lin, J. Chalmers, E. Ludlow, C. Pallaghy, G. Schrauf, Pablo Rush, A.M. Garcia, A. Mouradov and G.C. Spangenberg; Genetic transformation of rhodesgrass (Chloris gayana Kunth.) by particle bombardment 230; J. Matsumoto, S. Tsuruta, T. Gondo and R. Akashi; Modulation of the gibberellin content in transgenic turf-type bahiagrass for improved turf characteristics and reduced mowing requirements 231; F. Altpeter, M. Agharkar and H. Zhang; Inducible over-expression of the CBF3 abiotic stress regulon in transgenic bahiagrass (Paspalum notatum Flugge) 232; V.A. James and F. Altpeter; Genetic engineering for breeding for drought resistance and salt tolerance in Agropyron spp. (wheatgrass) 233; M. Fugui, Y. Jinfeng and H. Xiuwen; A novel genotype independent protocol for in vitro plant regeneration from mature seed derived callus of tall fescue (Festuca arundinacea Schreb.) 234; S. Chennareddy, R.V. Sairam and S.L. Goldman; Efficient in vitro regeneration system from seed derived callus of perennial ryegrass (Lolium perenne) and annual ryegrass (Lolium multiflorum) 235; S. Chennareddy, R.V. Sairam and S.L. Goldman; Nylon mesh as an improved support for bombarded calli or cell suspensions 236; S.J. Dalton, P. Robson, M. Buanafina, A.J.E. Bettany, E. Timms, D. Wiffen and P. Morris; A comparison of hygromycin and paromomycin selection strategies in the genetic transformation of seven Lolium, Festuca, Poa, and Agrostis species 237; S.J. Dalton, P. Robson, M. Buanafina, A.J.E. Bettany, E. Timms and P. Morris; Agrobacterium tumefaciens-mediated transformation of perennial ryegrass (Lolium perenne L.) 238; H. Sato, M. Fujimori, Y. Mano, T. Kiyoshi and T. Takamizo. Manipulating the phenolic acid content and digestibility of forage grasses by targeted expression of fungal cell wall degrading enzymes 239; M.M. de O. Buanafina, P. Morris, T. Langdon, S. Dalton, B. Hauck and H. Porter; Improving forage quality of tall fescue (Festuca arundinacea) by genetic manipulation of lignin biosynthesis 240; Z.-Y. Wang, L. Chen, C.-K. Auh, A. Hopkins and P. Dowling; Crown rust resistance in transgenic Italian ryegrass (L. multiflorum) expressing a rice chitinase gene and crosses with cytoplasmic male sterile hybrid ryegrass 241; W. Takahashi, M. Fujimori, Y. Miura, T. Komatsu, S. Sugita, A. Arakawa, Y. Nishizawa, H. Sato, Y. Mano, T. Hibi and T. Takamizo; Discovery, isolation and characterisation of promoters from perennial ryegrass (Lolium perenne) 242; A. Lidgett, N. Petrovska, J. Chalmers, N. Cummings and G.C. Spangenberg; Development and field evaluation of transgenic ryegrass (Lolium spp.) with down-regulation of main pollen allergens 243; N. Petrovska, A. Mouradov, Z.Y. Wang, K.F. Smith and G.C. Spangenberg; Shutting the stable door after the horse has bolted? Risk assessment and regulation for transgenic forages 244; C.J. Pollock; Assessing the risk posed by transgenic virus-resistant Trifolium repens to native grasslands in Southeast Australia 245; R.C. Godfree, P.W.G. Chu and A.G. Young; Pollen-mediated gene flow from genetically modified herbicide resistant creeping bentgrass 246; L.S. Watrud, E.H. Lee, A. Fairbrother, C. Burdick, J.R. Reichman, M. Bollman, M. Storm, G. King and P.K. van de Water; Use of cellular automata modelling approaches to understand potential impacts of GM grasses on grassland communities 247; R. Colasanti, R. Hunt and L.S. Watrud; Section 8: Genetic diversity, genetic resources and breeding systems 249; The importance of exotic forage germplasm in feeding New Zealand's livestock 251; J. Lancashire; Application of molecular diversity in a forage grass breeding program 252; A.A. Hopkins and M.C. Saha; Databases for managing genetic resources collections and mapping populations of forage and related species 253; I. Thomas, H. Ougham and D. Peltier; The use of molecular markers in genetic variability analysis of a collection of Dactylis glomerata L. 254; R. Costa, G. Pereira, C. Vicente and M.M. Tavares de Sousa; Genetic diversity in colonial bentgrass (Agrostis capillaris L.) revealed by EcoRI/MseI and PstI/MseI AFLP markers 256; H. Zhao and S. Bughrara; Genetic diversity in zoysiagrass ecotypes based on morphological characteristics and SSR markers 257; M. Hashiguchi, S. Tsuruta, T. Matsuo, M. Ebina, M. Kobayashi, H. Akamine and R. Akashi; Utilization of SSR to distinguish alfalfa cultivars 258; G.R. Bauchan, C. He and Z-L. Xia; Genetic diversity among alfalfa cultivars using SSR markers 259; S. Flajoulot, J. Ronfort, P. Baudouin, T. Huguet, P. Barre, C. Huyghe and B. Julier; Evaluation of genetic diversity in white clover (Trifolium repens L.) through measurement of simple sequence repeat (SSR) polymorphism 260; J. George, E. van Zijll de Jong, T.C. Wilkinson, M.P. Dobrowolksi, N.O.I. Cogan, K.F. Smith and J.W. Forster; Genetic and phenotypic diversity of Swiss red clover landraces 261; D. Herrmann, B. Boller, F. Widmer and R. Kolliker; Improving the utilisation of germplasm of Trifolium spumosum L. by the development of a core collection using ecogeographical and molecular techniques 262; K. Ghamkhar, R. Snowball and S.J. Bennett. Molecular characterization and tissue culture regeneration ability of the USA Arachis pintoi (Krap. and Greg.) germplasm collection 263; M.A. Carvalho, K.H. Quesenberry and M. Gallo-Meagher; Genetic and molecular characterization of temperate and tropical forage maize inbred lines 264; B. Alarcon-Zuniga, E. Valadez-Moctezuma, T. Cervantes-Martinez, T. Cervantes-Santana and M. Mendoza; Random amplified polymorphic DNA analysis in section Pnigma of the genus Bromus L. 265; M. Tuna, O. Barzani, K.P. Vogel and A. Golan-Goldhirsh; Genetic characterization of prairie grass (Bromus catharticus Vahl.) natural populations 266; R. Sellaro, E.M. Pagano, B. Rosso, P. Rimieri and R.D. Rios; Analysis of Bromus inermis populations using Amplified Fragment Length Polymorphism markers to identify duplicate accessions 267; V.L. Bradley and T.J. Kisha; Characterisation of naturalised populations of Thinopyrum ponticum Podp through indexes obtained under saline stress 268; S.M. Pistorale, A.N. Andres and O. Bazzigalupi; Genetic structure of Mongolian Wheatgrass (Agroypron mongolicum Keng) in Inner Mongolia of China 269; Y. Jinfeng, Z. Mengli and X. Xinmin; RFLP analyses of chloroplast DNA of the crested wheatgrasses 270; K.P. Vogel, D.J. Lee and C.A. Caha; Tracing the origins of Timothy species (Phleum sp.) 271; A.V. Stewart, A. Joachimiak and N. Ellison; Genetic diversity and heterosis in perennial ryegrass 272; U.K. Posselt; Population genetics of perennial ryegrass (Lolium perenne L.): differentiation of pasture and turf cultivars 273; M.P. Dobrowolski, N.R. Bannan, R.C. Ponting, J.W. Forster and K.F. Smith; Analysis of genetic changes in single-variety ryegrass swards 274; C. Straub, G. Boutet and C. Huyghe; Genetic variability between adapted populations of annual ryegrass (Lolium multiflorum Lam) in Argentina 275; A. Andres. B. Rosso, J. De Battista and M. Acuna; Does AFLP diversity reflect consanguinity within meadow fescue breeding material? 276; B. Boller and R. Kolliker; Genetic diversity in Festuca species as shown by AFLP 277; X.Q. Zhang and S.S. Bughrara; Analyses of genetic change in grass-clover based systems over time 278; A. Ghesquiere, K. Mehdikhanlov, M. Malengier and J. De Riek; Keyword index 279; Author index 283.