Unnatural amino acids : methods and protocols
Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of unnatural amino acids in peptides with antimicrobial properties, genetic incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular Biology"!series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids
eBook, English, ©2012
Humana Press ; Springer Science+Business Media, LLC, [Totowa, NJ], New York, ©2012
Methods in molecular biology (Clifton, N.J.), v. 794, 794
Internet Resources
1 online resource (xiv, 409 pages) : illustrations
9781617793318, 9781617793301, 1617793310, 1617793302
756512314
Printed edition:
Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases
Multistep enzyme catalyzed reactions for unnatural amino acids
Enzymatic production of enantiopure amino acids from mono-substituted hydantoin substrates
Preparation of glutamate analogues by enzymatic transamination
Carbon-carbon bond-forming enzymes for the synthesis of non-natural amino acids
Engineering cyclic amidases for non-natural amino acid synthesis
NMR analysis of unnatural amino acids in natural antibiotics
Site-specific incorporation of unnatural amino acids as probes for protein conformational changes
Application of unnatural amino acids to the de novo design of selective antibiotic peptides
Use of unnatural amino acids to probe structure-activity relationships and mode-of-action of antimicrobial peptides
Experimental methods for scanning unnatural amino acid mutagenesis
Genetic incorporation of unnatural amino acids into proteins in yeast
Site-specific incorporation of unnatural amino acids into proteins in mammalian cells
Incorporation of unnatural non-alpha-amino acids into the N-terminus of proteins in a cell-free translation system
Site-specific modification of proteins by the staudinger-phosphite reaction
Hplc methods for determination of D-aspartate and N-methyl-D-aspartate
Estimation of chronological age from the racemization rate of L- and D-aspartic acid: How to completely separate enantiomers from dentin
Enzymatic detection of D-amino acids
An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures
Electrophysiological analysis of the modulation of NMDA-receptors function by D-serine and glycine in the central nervous system
Biosensors for d-amino acid detection
Analysis of D-[beta]-aspartyl isomers at specific sites in proteins
Nutritional value of D-amino acids, d-peptides, and amino acid derivatives in mice
Preparation and assay of recombinant serine racemase
Assay of amino acid racemases
Assays of D-amino acid oxidases
Enzymes acting on D-amino acid containing peptides. Preparation of Unnatural Amino Acids with Ammonia-lyases and 2,3-aminomutases
Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids
Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates
Preparation of Glutamate Analogues by Enzymatic Transamination
Carbon-Carbon Bond Forming Enzymes for the Synthesis of Non-natural Amino Acids.- Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis
NMR Analysis of Unnatural Amino Acids in Natural Antibiotics
Site-specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes
Application of Unnatural Amino Acids to the De novo Design of Selective Antibiotic Peptides
Use of Unnatural Amino Acids to Probe Structure-activity Relationships and Mode-of-action of Antimicrobial Peptides
Experimental Methods for Scanning Unnatural Amino Acid Mutagenesis
Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast
Site-specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells
Incorporation of Unnatural Non-a-amino Acids into the N-terminus of Proteins in a Cell-free Translation System
Site Specific Modification of Proteins by the Staudinger-phosphite Reaction
HPLC Methods for Determination of D-aspartate and N-methyl-D-aspartate
Estimation of Chronological Age from the Racemization Rate of L- and D-aspartic Acid: How to Completely Separate Enantiomers from Dentin
Enzymatic Detection of D-amino Acids
An Enzymatic-HPLC Assay to Monitor Endogenous D-serine Release from Neuronal Cultures
Electrophysiological Analysis of the Modulation of NMDA-receptors Function by d-serine and Glycine in the Central Nervous System
Biosensors for D-amino Acid Detection
Analysis of D-β-aspartyl Isomers at Specific Sites in Proteins
Nutritional Value of D-amino Acids, D-peptides and Amino Acid Derivatives in Mice
Preparation and Assay of Recombinant Serine Racemase
Assay of Amino Acid Racemases
Assays of D-amino Acid Oxidases
Enzymes Acting on D-amino Acid Containing Peptides
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