The nutritional role of acetate for lactic acid bacteria
Beverly Marie Guirard (Author), Roger J. Williams (Degree supervisor)
"As one approach to elucidation of the role of acetate in the metabolism of lactic acid bacteria, all of these substances reportedly formed from acetate or capable of degradation to acetate have been tested for their ability to duplicate the growth effect of acetate for various representative organisms of this group. In addition, certain intermediates known to be involved in carbohydrate and fat metabolism were investigated. Along with the pure substances, some cellular and tissue extracts were tested. Many of these were more active in promoting growth than acetate itself per unit weight. Consequently, the concentration of the active factor (or factors) in the most promising source materials was undertaken. The nature of the active material(s) in cellular and tissue extracts, details of the fractionation procedures, and the extent of concentration effected are given in the text of the thesis. Another approach toward ascertaining the nutritional role of sodium acetate for lactic acid bacteria was attempted. The bacterial cells grown in a complete medium were saponified, and the fatty acid and unsaponifiable fractions were isolated and tested as growth supplements for these organisms. These experiments yielded information which is concordant with the view that acetate is utilized by lactic acid bacteria in the synthesis of lipoid and steroid materials"--Leaves 10-11
Thesis, Dissertation, English, 1945