Freshney's culture of animal cells : a manual of basic technique and specialized applications
Amanda Capes-Davis (Author), R. Ian Freshney
"Cell culture is the process used to keep cells alive in the laboratory. The process has undergone many changes since its discovery in 1907, as better techniques, equipment, and reagents continue to be developed. Cell culture now relies on a toolkit of techniques (e.g. aseptic technique), equipment (e.g. biosafety cabinet), and reagents (e.g. flasks and growth medium). Cells can be maintained and grown from many species and tissues, ranging from humans to fish, and from skin to brain. New methods and uses are emerging, allowing scientists to grow stem cells and develop more effective models through three-dimensional culture. However, cell culture can be challenging. Problems can affect any cell culture practitioner, even the most seasoned expert. Training is needed to ensure aseptic conditions, skilled handling, and an awareness of safety issues and of the purity and identity of the material being handled"-- Provided by publisher
eBook, English, 2021
Eighth edition
Wiley-Blackwell, Hoboken, NJ, 2021