Carbon isotope fractionation by ribulose 1,5-bisphosphate carboxylase from various organisms
Marilyn Louise Fogel Estep (Author)
"This dissertation reports reproducible carbon isotope fractionations by structurally and catalytically distinct RuBP carboxylases from several sources: spinach leaves, the blue-green alga Agmenellum quadruplicatum strain PR-6; two purple, non-sulfur, photosynthetic bacteria, Rhodospirillum rubrum and Rhodopseudomonas sphaeroides; a chemosynthetic bacterium Hydrogenomonas eutropha; and a marine, pennate diatom Cylindrotheca sp. strain N-1. Reaction conditions were slightly modified to test their effects on the carbon isotope fractionation value: temperature, oxygen level, NaHCO₃ levels, buffer, pH, presence of 6-phosphogluconate, metal cofactors, substrate, and enzyme of differing purity from the same source. In addition, isolation and characterization of RuBP carboxylase from Cylindrotheca sp. (Morgan) strain N-1 is reported"--Leaf 25
Thesis, Dissertation, English, 1977
1977