Enzyme activities in the fat body of the tsetse fly Glossina morsitans and the fleshfly Sarcophaga tibialis in relation to proline metabolism
Enzymes were assayed in the fat body of the tsetse fly Glossina morsitans and compared with those in the fat body of the fleshfly Sarcophaga tibialis. Alanine aminotransferase, 3-hydroxyacyl-CoA dehydrogenase, NADP-linked isocitrate dehydrogenase and proline synthetase were much more active in G. morsitans. NADP-linked malic enzyme, pyruvate carboxylase, citrate synthase and aconitase were also more active in G. morsitans, but α-oxoglutarate dehydrogenase, fumarase, malate dehydrogenase and various glycolytic enzymes were more active in S. tibialis. Pyruvate carboxylase was mainly mitochondrial in the fat body of both insects, whereas NADP-linked malic enzyme was cytoplasmic. Alanine aminotransferase and NADP-linked isocitrate dehydrogenase were more predominantly mitochondrial in G. morsitans than in S. tibialis. In the fat body of G. morsitans the K
Article
Insect Biochemistry, 14, 1984, 685