In vitro growth responses and plant regeneration from cryopreserved meristems of cassava (Manihot esculenta Crantz)*)
A droplet-freezing method has been developed for the cryopreservation of cassava, Manihot esculenta Crantz, meristems. The meristems treated with a 15% DMSO + 3% sucrose solution for 15 min were frozen over an 18 microm aluminium foil in 2-3 microl droplets of the cryoprotectants in plastic petri dishes at a cooling rate of 0.5 grausC/ min to various sub-zero temperatures (-20;-25;-30 and -40 grausC) and stored in liquid nitrogen (-196 grausC). The meristems retrieved from liquid nitrogen storage, upon thawing and return to in vitro culture on plant regeneration medium, exhibited various morphogenetic responses such as differentiation of callus and leaves and whole plantlets. The were successfully grown in pots
Article, English, 1982