Front cover image for Introducing mothur : open-source, platform-independent, community-supported software for describing and comparing microbial communities

Peer-reviewed

Introducing mothur : open-source, platform-independent, community-supported software for describing and comparing microbial communities

Patrick D. Schloss (Author), Sarah L. Westcott (Author), Thomas Ryabin (Author), Justine R. Hall (Author), Martin Hartmann (Author), Emily B. Hollister (Author), Ryan A. Lesniewski (Author), Brian B. Oakley (Author), Donovan H. Parks (Author), Courtney J. Robinson (Author), Jason W. Sahl (Author), Blaž. Stres (Author), Gerhard G. Thallinger (Author), David J. van Horn (Author), Caroly F. Weber (Author)
Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur totrim, screen, and align sequences, calculate distances, assign sequences to OTUs, and describe that alfa-and ß-diversity of eight marine samples previously characterized by pyrpsequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 hours using laptop computer

Downloadable Article, English, 2009